Human PCK2(Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) ELISA Kit

Manual
  • Specifications
Product name

Human PCK2(Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) ELISA Kit

Catalogue No.

EH1163

Alias

PEPCK-M(Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) ELISA Kit, PEPCK2 ELISA Kit, PCK2 ELISA Kit, Phosphoenolpyruvate carboxylase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PCK2 ELISA Kit allows for the in vitro quantitative determination of PCK2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PCK2(Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PCK2 and the recovery rates were calculated by comparing the measured value to the expected amount of PCK2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-101 96
EDTA plasma(n=5) 85-98 93
heparin plasma(n=5) 89-98 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 88-104% 85-99% 87-102%
EDTA plasma(n=5) 82-100% 87-99% 87-96% 83-96%
heparin plasma(n=5) 80-93% 81-98% 81-87% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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