Human IL-28A(Interleukin-28A) ELISA Kit

Manual
  • Specifications
Product name

Human IL-28A(Interleukin-28A) ELISA Kit

Catalogue No.

EH0194

Alias

IL-28A ELISA Kit, Cytokine Zcyto20 ELISA Kit, Interferon lambda-2(IFN-lambda-2) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-28A ELISA Kit allows for the in vitro quantitative determination of IL-28A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human IL-28A(Interleukin-28A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-28A and the recovery rates were calculated by comparing the measured value to the expected amount of IL-28A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-105 95
EDTA plasma(n=5) 88-97 92
heparin plasma(n=5) 85-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-28A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 86-100% 85-105% 89-100%
EDTA plasma(n=5) 84-101% 82-95% 82-101% 82-100%
heparin plasma(n=5) 80-96% 84-96% 86-96% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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