Human IDH2(Isocitrate dehydrogenase [NADP], mitochondrial) ELISA Kit

Manual
  • Specifications
Product name

Human IDH2(Isocitrate dehydrogenase [NADP], mitochondrial) ELISA Kit

Catalogue No.

EH1339

Alias

IDH2 ELISA Kit, Isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit, ICD-M ELISA Kit, IDH ELISA Kit, IDHM ELISA Kit, IDP ELISA Kit, mNADP-IDH ELISA Kit, Oxalosuccinate decarboxylase ELISA Kit, NADP(+)-specific ICDH ELISA Kit, ICD-M ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IDH2 ELISA Kit allows for the in vitro quantitative determination of IDH2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human IDH2(Isocitrate dehydrogenase [NADP], mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IDH2 and the recovery rates were calculated by comparing the measured value to the expected amount of IDH2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 94
EDTA plasma(n=5) 91-104 98
heparin plasma(n=5) 94-104 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDH2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 85-105% 90-105% 88-101%
EDTA plasma(n=5) 92-98% 82-101% 82-100% 87-96%
heparin plasma(n=5) 89-100% 84-100% 80-96% 80-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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