Human HNRNPF(Heterogeneous nuclear ribonucleoprotein F) ELISA Kit

Manual
  • Specifications
Product name

Human HNRNPF(Heterogeneous nuclear ribonucleoprotein F) ELISA Kit

Catalogue No.

EH1322

Alias

HNRNPF ELISA Kit, Heterogeneous nuclear ribonucleoprotein F ELISA Kit, hnRNP F ELISA Kit, Nucleolin-like protein mcs94-1 ELISA Kit, HNRPF ELISA Kit, OK ELISA Kit, SW-cl.23 ELISA Kit, mcs94-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HNRNPF ELISA Kit allows for the in vitro quantitative determination of HNRNPF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human HNRNPF(Heterogeneous nuclear ribonucleoprotein F) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HNRNPF and the recovery rates were calculated by comparing the measured value to the expected amount of HNRNPF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 93-98 95
EDTA plasma(n=5) 85-103 91
heparin plasma(n=5) 86-105 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HNRNPF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 89-103% 91-101% 85-100%
EDTA plasma(n=5) 88-101% 86-101% 82-98% 89-101%
heparin plasma(n=5) 82-98% 81-98% 83-92% 84-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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