Human GLUD1(Glutamate dehydrogenase 1, mitochondrial) ELISA Kit

Manual
  • Specifications
Product name

Human GLUD1(Glutamate dehydrogenase 1, mitochondrial) ELISA Kit

Catalogue No.

EH0825

Alias

GLUD1(Glutamate dehydrogenase 1, mitochondrial) ELISA Kit, GDH 1 ELISA Kit, GLUD ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

GLUD1 ELISA Kit allows for the in vitro quantitative determination of GLUD1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40mIU/ml

Sensitivity

< 0.375mIU/ml

Standard curve

Human GLUD1(Glutamate dehydrogenase 1, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GLUD1 and the recovery rates were calculated by comparing the measured value to the expected amount of GLUD1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 93-104 99
EDTA plasma(n=5) 88-100 92
heparin plasma(n=5) 87-98 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GLUD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 87-103% 88-105% 87-100%
EDTA plasma(n=5) 82-94% 84-98% 82-99% 82-96%
heparin plasma(n=5) 85-93% 83-96% 83-100% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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