Human COX5A(Cytochrome c oxidase subunit 5A, mitochondrial) ELISA Kit

Manual
  • Specifications
Product name

Human COX5A(Cytochrome c oxidase subunit 5A, mitochondrial) ELISA Kit

Catalogue No.

EH1130

Alias

COX5A ELISA Kit, Cytochrome c oxidase subunit 5A, mitochondrial ELISA Kit, Cytochrome c oxidase polypeptide Va ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

COX5A ELISA Kit allows for the in vitro quantitative determination of COX5A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human COX5A(Cytochrome c oxidase subunit 5A, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of COX5A and the recovery rates were calculated by comparing the measured value to the expected amount of COX5A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-102 93
EDTA plasma(n=5) 85-100 92
heparin plasma(n=5) 88-101 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COX5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-102% 97-104% 86-104% 88-100%
EDTA plasma(n=5) 84-99% 88-95% 82-92% 82-88%
heparin plasma(n=5) 83-96% 82-100% 84-98% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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