Human ATOH1(Protein atonal homolog 1) ELISA Kit

Manual
  • Specifications
Product name

Human ATOH1(Protein atonal homolog 1) ELISA Kit

Catalogue No.

EH0804

Alias

ATOH1(Protein atonal homolog 1) ELISA Kit, ATH1 ELISA Kit, Class A basic helix-loop-helix protein 14(bHLHa14) ELISA Kit, Helix-loop-helix protein hATH-1(hATH1) ELISA Kit, BHLHA14 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ATOH1 ELISA Kit allows for the in vitro quantitative determination of ATOH1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human ATOH1(Protein atonal homolog 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ATOH1 and the recovery rates were calculated by comparing the measured value to the expected amount of ATOH1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-96 91
EDTA plasma(n=5) 91-100 96
heparin plasma(n=5) 94-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATOH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 85-104% 85-105% 87-103%
EDTA plasma(n=5) 83-100% 86-101% 90-101% 84-99%
heparin plasma(n=5) 87-97% 80-100% 82-100% 84-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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