Human AKAP17A(A-kinase anchor protein 17A) ELISA Kit

Manual
  • Specifications
Product name

Human AKAP17A(A-kinase anchor protein 17A) ELISA Kit

Catalogue No.

EH1213

Alias

AKAP17A ELISA Kit, A-kinase anchor protein 17A ELISA Kit, AKAP-17A ELISA Kit, Splicing factor, arginine ELISA Kit, serine-rich 17A ELISA Kit, Protein kinase A-anchoring protein 17A ELISA Kit, Protein XE7 ELISA Kit, 721P ELISA Kit, B-lymphocyte antigen ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

AKAP17A ELISA Kit allows for the in vitro quantitative determination of AKAP17A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human AKAP17A(A-kinase anchor protein 17A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AKAP17A and the recovery rates were calculated by comparing the measured value to the expected amount of AKAP17A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-102 95
EDTA plasma(n=5) 91-98 96
heparin plasma(n=5) 86-101 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKAP17A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 87-101% 89-104% 87-105%
EDTA plasma(n=5) 82-100% 84-95% 83-97% 91-99%
heparin plasma(n=5) 92-100% 84-99% 87-99% 81-88%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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