Human ABCB5(ATP-binding cassette sub-family B member 5) ELISA Kit

Manual
  • Specifications
Product name

Human ABCB5(ATP-binding cassette sub-family B member 5) ELISA Kit

Catalogue No.

EH1172

Alias

ABCB5(ATP-binding cassette sub-family B member 5) ELISA Kit, ABCB5 P-gp ELISA Kit, ABCB5alpha ELISA Kit, ABCB5beta ELISA Kit, ATP-binding cassette protein ELISA Kit, ATP-binding cassette, sub-family B(MDR ELISA Kit, TAP), member 5 ELISA Kit, P-glycoprotein ABCB5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ABCB5 ELISA Kit allows for the in vitro quantitative determination of ABCB5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human ABCB5(ATP-binding cassette sub-family B member 5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ABCB5 and the recovery rates were calculated by comparing the measured value to the expected amount of ABCB5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-103 95
EDTA plasma(n=5) 94-103 98
heparin plasma(n=5) 88-97 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ABCB5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-99% 97-105% 91-105% 86-103%
EDTA plasma(n=5) 89-99% 82-96% 82-93% 87-96%
heparin plasma(n=5) 81-93% 80-97% 84-99% 81-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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