- Catalogue No.
K134
- Size
48T(48S)/96T(96S)
- Range
0.0313-2mM
- Sensitivity
0.0156mM
- Kit component
|
Reagents
|
48T
|
96T
|
Storage
|
|
Cu Reagent
|
6 mL
|
12 mL
|
2-8°C protected from light
|
|
Chromogen
|
15 mL
|
30 mL
|
2-8°C protected from light
|
|
Standard
(16.41 mg Palmitic Acid)
|
Powder×1 vial
|
Powder×1 vial
|
2-8°C protected from light
|
- Storage
The kit should be stored at 2-8℃ shading light for one year.
- Application
This kit can be used to measure the content of free fatty acids in biological samples, including serum (plasma), animal and plant tissues, cells and bacteria.
- Principle of the Assay
Free fatty acids (FFA), also known as non-esterified fatty acids, are both the products of fat hydrolysis and the substrates for fat synthesis, and they circulate in the plasma bound to albumin. The concentration of FFA in serum is related to lipid metabolism, glucose metabolism, and endocrine function. The concentration of FFA will increase due to diseases such as diabetes, severe liver disorders, and hyperthyroidism.
The principle of Free Fatty Acids (FFA) Assay Kit((Microplate Method)) is that FFA combines with copper ions to form copper fatty acid salts, which dissolve in chloroform. The content of copper ions is determined by the copper reagent method, and the content of free fatty acids can be calculated accordingly.
- Materials Not Supplied
microplate reader or visible spectrophotometer(capable of measuring absorbance at 550nm), incubator, ice maker, low-temperature centrifuge
96-well plates or micro glass cuvettes, adjustable pipettes and pipette tips, homogenizer (for tissue samples), glass bottles (for preparing extraction solution)
n-heptane, anhydrous methanol, chloroform (trichloromethane)
- Notes
1. This product is for scientific research use by professionals only.
2. Please pay attention to safety precautions and follow the laboratory reagent operation norms. For your safety and health, please wear a lab coat and disposable gloves during the operation.
