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ERCC2 antibody

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Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Synonyms：	General transcription and DNA repair factor IIH helicase subunit XPD (TFIIH subunit XPD)\|Basic transcription factor 2 80 kDa subunit (BTF2 p80)\|CXPD\|DNA excision repair protein ERCC-2\|DNA repair protein complementing XP-D cells\|TFIIH basal transcription factor complex 80 kDa subunit (TFIIH 80 kDa subunit antibody, TFIIH p80)\|Xeroderma pigmentosum group D-complementing protein\|ERCC2\|XPD\|XPDC antibody
Catalogue No.：	FNab10932	Reactivity：	Human, Mouse, Rat
Host：	Rabbit	Tested Application：	ELISA, WB
Clonality：	polyclonal	Isotype：	IgG

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SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: ERCC2 antibody
Catalogue No.: FNab10932
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: excision repair cross-complementing rodent repair deficiency, complementation group 2
Alternative Names: General transcription and DNA repair factor IIH helicase subunit XPD (TFIIH subunit XPD)|Basic transcription factor 2 80 kDa subunit (BTF2 p80)|CXPD|DNA excision repair protein ERCC-2|DNA repair protein complementing XP-D cells|TFIIH basal transcription factor complex 80 kDa subunit (TFIIH 80 kDa subunit antibody, TFIIH p80)|Xeroderma pigmentosum group D-complementing protein|ERCC2|XPD|XPDC antibody
UniProt ID: P18074
Observed MW: 80 kDa

Application

Tested Applications: ELISA, WB
Recommended dilution: WB: 1:500-1:2000

Validated Images

Various lysates were subjected to SDS PAGE followed by western blot with FNab10932(ERCC2 antibody) at dilution of 1:1000

Background: ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair(NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase(CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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