Products

anti- USP10 antibody

FNab10128 Catalogue No.
Synonyms
Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Tested Application
ELISA, IHC, IF
Product Information
Product Name
anti- USP10 antibody
Catalogue No.
FNab10128
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989).
Immunogen Information
Immunogen
ubiquitin specific peptidase 10
Synonyms
Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10
Application
Tested Application
ELISA, IHC, IF
Recommended dilution
IHC: 1:50-1:200; IF: 1:20-1:100
Uniprot information
UniProt ID
Q14694
Images
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab10128 (USP10 Antibody) at dilution of 1:100.
Immunofluorescence analysis of A549 cells using USP10 antibody (FNab10128). Blue: DAPI for nuclear staining.