NPPA antibody

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Synonyms:Natriuretic peptides A|Atrial natriuretic factor prohormone (proANF)|Atrial natriuretic peptide prohormone (preproANP antibody, proANP)|Atriopeptigen|Cardiodilatin (CDD)|preproCDD-ANF|Long-acting natriuretic peptide (LANP) Alternative names: Long-acting natriuretic hormone (LANH)|Pro atrial natriuretic factor 1-30 (proANF 1-30)|Pro atrial natriuretic peptide 1-30 (proANP 1-30)|Vessel dilator (VSDL) Alternative names: Pro atrial natriuretic factor 31-67 (proANF 31-67)|Pro atrial natriuretic peptide 31-67 (proANP 31-67)|Kaliuretic peptide (KP) Alternative names: Pro atrial natriuretic factor 79-98 (proANF 79-98)|Pro atrial natriuretic peptide 79-98 (proANP 79-98)|Urodilatin (URO) Alternative names: CDD 95-126|CDD-ANP (95-126)|Pro atrial natriuretic peptide 95-126 (proANP 95-126)|Auriculin-C Alternative names: Atrial natriuretic factor 1-33 (ANF 1-33)|Auriculin-D Alternative names: Atrial natriuretic factor 3-33 (ANF 3-33)|Atrial natriuretic peptide (ANP) Alternative names: Alpha-atrial natriuretic peptide|Alpha-hANP|Atrial natriuretic factor (ANF)|CDD-ANF|CDD-ANP (99-126)|Cardionatrin|Pro atrial natriuretic factor 99-126 (proANF 99-126)|Auriculin-B Alternative names: Atrial natriuretic factor 8-33 (ANF 8-33)|Auriculin-A|Atriopeptin-1 Alternative names: Atriopeptin I|Atriopeptin-2 Alternative names: Atriopeptin II|Atriopeptin-3 Alternative names: Atriopeptin III|NPPA|ANP|PND antibody
Catalogue No.:FNab09839Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
Size Price
100ug Inquiry
Dispatch Time: About 3 working days
  • SPECIFICATIONS
  • FIGURES
  • CONDITIONS
  • FAQS
Product Name
NPPA antibody
Catalogue No.
FNab09839
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
natriuretic peptide precursor A
Alternative Names
Natriuretic peptides A|Atrial natriuretic factor prohormone (proANF)|Atrial natriuretic peptide prohormone (preproANP antibody, proANP)|Atriopeptigen|Cardiodilatin (CDD)|preproCDD-ANF|Long-acting natriuretic peptide (LANP) Alternative names: Long-acting natriuretic hormone (LANH)|Pro atrial natriuretic factor 1-30 (proANF 1-30)|Pro atrial natriuretic peptide 1-30 (proANP 1-30)|Vessel dilator (VSDL) Alternative names: Pro atrial natriuretic factor 31-67 (proANF 31-67)|Pro atrial natriuretic peptide 31-67 (proANP 31-67)|Kaliuretic peptide (KP) Alternative names: Pro atrial natriuretic factor 79-98 (proANF 79-98)|Pro atrial natriuretic peptide 79-98 (proANP 79-98)|Urodilatin (URO) Alternative names: CDD 95-126|CDD-ANP (95-126)|Pro atrial natriuretic peptide 95-126 (proANP 95-126)|Auriculin-C Alternative names: Atrial natriuretic factor 1-33 (ANF 1-33)|Auriculin-D Alternative names: Atrial natriuretic factor 3-33 (ANF 3-33)|Atrial natriuretic peptide (ANP) Alternative names: Alpha-atrial natriuretic peptide|Alpha-hANP|Atrial natriuretic factor (ANF)|CDD-ANF|CDD-ANP (99-126)|Cardionatrin|Pro atrial natriuretic factor 99-126 (proANF 99-126)|Auriculin-B Alternative names: Atrial natriuretic factor 8-33 (ANF 8-33)|Auriculin-A|Atriopeptin-1 Alternative names: Atriopeptin I|Atriopeptin-2 Alternative names: Atriopeptin II|Atriopeptin-3 Alternative names: Atriopeptin III|NPPA|ANP|PND antibody
UniProt ID
P01160
Observed MW
17 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09839( NPPA Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat heart tissue slide using FNab09839( NPPA Antibody) at dilution of 1:200
Background
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.
How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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