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TNF alpha antibody

Category:

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	Tumor necrosis factor\|Cachectin\|TNF-alpha\|Tumor necrosis factor ligand superfamily member 2 (TNF-a)\|Tumor necrosis factor antibody, membrane form Alternative names: N-terminal fragment (NTF)\|Intracellular domain 1 (ICD1)\|Intracellular domain 2 (ICD2)\|C-domain 1\|C-domain 2\|Tumor necrosis factor antibody, soluble form\|TNF\|TNFA\|TNFSF2 antibody
Catalogue No.：	FNab08815	Reactivity：	Human, Mouse, Rat
Host：	Mouse	Tested Application：	ELISA, WB, IHC
Clonality：	monoclonal	Isotype：	IgG2b

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: TNF alpha antibody
Catalogue No.: FNab08815
Size: 100μg
Form: liquid
Purification: Protein A+G purification
Purity: ≥95% as determined by SDS-PAGE
Clonality: monoclonal
Isotype: IgG2b
Clone ID: 2A9
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: tumor necrosis factor(TNF superfamily, member 2)
Alternative Names: Tumor necrosis factor|Cachectin|TNF-alpha|Tumor necrosis factor ligand superfamily member 2 (TNF-a)|Tumor necrosis factor antibody, membrane form Alternative names: N-terminal fragment (NTF)|Intracellular domain 1 (ICD1)|Intracellular domain 2 (ICD2)|C-domain 1|C-domain 2|Tumor necrosis factor antibody, soluble form|TNF|TNFA|TNFSF2 antibody
UniProt ID: P01375
Observed MW: 22 kDa

Application

Tested Applications: ELISA, WB, IHC
Recommended dilution: WB: 1:500-1:5000; IHC: 1:200-1:1000

Validated Images

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with FNab08815(TNF-a Antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08815(TNF-a Antibody) at dilution of 1:800. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Background: TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme(TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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