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Spast antibody

Category:

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	Spastin\|Spast\|Kiaa1083\|Spg4 antibody
Catalogue No.：	FNab08156	Reactivity：	Human, Mouse, Rat
Host：	Rabbit	Tested Application：	ELISA, WB
Clonality：	polyclonal	Isotype：	IgG

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: Spast antibody
Catalogue No.: FNab08156
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: spastin
Alternative Names: Spastin|Spast|Kiaa1083|Spg4 antibody
UniProt ID: Q9QYY8
Observed MW: 52 kDa

Application

Tested Applications: ELISA, WB
Recommended dilution: WB: 1:500-1:2000

Validated Images

PC-3 cells were subjected to SDS PAGE followed by western blot with FNab08156(SPAST antibody) at dilution of 1:1000

Background: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated(PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold(By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination(By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation(By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia(By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex(By similarity). Recruited at the midbody, probably by IST1, and participates to membrane fission during abscission together with the ESCRT-III complex(By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase(By similarity). Required for membrane traffic from the endoplasmic reticulum(ER) to the Golgi and endosome recycling(By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing(By similarity). Probably plays a role in axon growth and the formation of axonal branches(PubMed:18234839). Isoform 1: Involved in lipid metabolism by regulating the size and distribution of lipid droplets.

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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