Products
RXRA antibody
- Product Name
- RXRA antibody
- Catalogue No.
- FNab07542
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- retinoid X receptor, alpha
- Alternative Names
- Retinoic acid receptor RXR-alpha|Nuclear receptor subfamily 2 group B member 1|Retinoid X receptor alpha|RXRA|NR2B1 antibody
- UniProt ID
- P19793
- Observed MW
- 51 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab07542(RXRA antibody) at dilution of 1:600
IP Result of anti-RXRA (IP: FNab07542, 4ug; Detection: FNab07542 1:600) with MCF-7 cells lysate 800ug.
Immunohistochemistry of paraffin-embedded human kidney using FNab07542(RXRA antibody) at dilution of 1:200
Immunofluorescent analysis of Hela cells, using RXRA antibody FNab07542 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements(RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements(RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
