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RPS6KB1 antibody

Category:

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Synonyms：	Ribosomal protein S6 kinase beta-1 (S6K-beta-1 antibody, S6K1)\|70 kDa ribosomal protein S6 kinase 1 (P70S6K1 antibody, p70-S6K 1)\|Ribosomal protein S6 kinase I\|Serine/threonine-protein kinase 14A\|p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha antibody, p70 S6K-alpha antibody, p70 S6KA)\|RPS6KB1\|STK14A antibody
Catalogue No.：	FNab06090	Reactivity：	Human, Mouse, Rat
Host：	Rabbit	Tested Application：	ELISA, WB, IHC, IF, IP, FC
Clonality：	polyclonal	Isotype：	IgG

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: RPS6KB1 antibody
Catalogue No.: FNab06090
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: ribosomal protein S6 kinase, 70kDa, polypeptide 1
Alternative Names: Ribosomal protein S6 kinase beta-1 (S6K-beta-1 antibody, S6K1)|70 kDa ribosomal protein S6 kinase 1 (P70S6K1 antibody, p70-S6K 1)|Ribosomal protein S6 kinase I|Serine/threonine-protein kinase 14A|p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha antibody, p70 S6K-alpha antibody, p70 S6KA)|RPS6KB1|STK14A antibody
UniProt ID: P23443
Observed MW: 65-70 kDa

Application

Tested Applications: ELISA, WB, IHC, IF, IP, FC
Recommended dilution: WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:100-1:400; IF: 1:10-1:100

Validated Images

HeLa cells were subjected to SDS PAGE followed by western blot with FNab06090(p70(S6K) antibody) at dilution of 1:1200

IP Result of anti-p70S6K (IP:FNab06090, 3ug; Detection:FNab06090 1:1000) with HeLa cells lysate 3000ug.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06090(p70(S6K) Antibody) at dilution of 1:200

Immunofluorescent analysis of HepG2 cells using FNab06090 ( p70S6K Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background: RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway and it plays a key role in cell growth and proliferation by regulating insulin sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy.(PMID:22684641). It has 2 isoforms produced by alternative initiation.

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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