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NOTCH1 antibody

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	Neurogenic locus notch homolog protein 1 (Notch 1 antibody, hN1)\|Translocation-associated notch protein TAN-1\|Notch 1 extracellular truncation (NEXT)\|Notch 1 intracellular domain (NICD)\|NOTCH1\|TAN1 antibody
Catalogue No.：	FNab05799	Reactivity：	Human, Mouse, Rat
Host：	Rabbit	Tested Application：	ELISA, WB, IHC, IP
Clonality：	polyclonal	Isotype：	IgG

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
CITATIONS
FIGURES
CONDITIONS
FAQS

Product Name: NOTCH1 antibody
Catalogue No.: FNab05799
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: Notch homolog 1, translocation-associated(Drosophila)
Alternative Names: Neurogenic locus notch homolog protein 1 (Notch 1 antibody, hN1)|Translocation-associated notch protein TAN-1|Notch 1 extracellular truncation (NEXT)|Notch 1 intracellular domain (NICD)|NOTCH1|TAN1 antibody
UniProt ID: P46531
Observed MW: 270 kDa, 120kd

Application

Tested Applications: ELISA, WB, IHC, IP
Recommended dilution: WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:500

Validated Images

human brain tissue were subjected to SDS PAGE followed by western blot with FNab05799(NOTCH1 antibody) at dilution of 1:500

IP Result of anti-Notch1 (IP: FNab05799 4ug; Detection: FNab05799 1:200) with HeLa cells lysate 2000ug.

Immunohistochemistry of paraffin-embedded mouse brain tissue tissue slide using FNab05799(NOTCH1 Antibody) at dilution of 1:200

Background: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain(NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone(SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile(sensory) cilia at the left-right organiser(LRO).The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and all the three cleaved NOTCH1 forms 110-120 kDa.

Effect of humanine on the Notch signaling pathway in myocardial infarction

IF: 2.3

Journal:

Turkish Journal of Medical Sciences

Author:

Department of Medical Pharmacology, Faculty of Medicine, Ad?yaman University, Ad?yaman, Turkiye

Cited Date:

2023-12-29

Product:

Hydroxytyrosol protects isoproterenol-induced myocardial infarction through activating notch signaling

Journal:

Iranian Journal of Basic Medical Sciences

Author:

Department of Medical Pharmacology, Faculty of Medicine, Ad?yaman University, Ad?yaman, 02040, Turkey.

Sample:

heart tissues

Cited Date:

2025-02-07

Product:

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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