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MTOR antibody

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	Serine/threonine-protein kinase mTOR\|FK506-binding protein 12-rapamycin complex-associated protein 1\|FKBP12-rapamycin complex-associated protein\|Mammalian target of rapamycin (mTOR)\|Mechanistic target of rapamycin\|Rapamycin and FKBP12 target 1\|Rapamycin target protein 1\|MTOR\|FRAP\|FRAP1\|FRAP2\|RAFT1\|RAPT1 antibody
Catalogue No.：	FNab05417	Reactivity：	Human, Mouse, Rat
Host：	Rabbit	Tested Application：	ELISA, WB, IHC
Clonality：	polyclonal	Isotype：	IgG

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
CITATIONS
FIGURES
CONDITIONS
FAQS

Product Name: MTOR antibody
Catalogue No.: FNab05417
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: FK506 binding protein 12-rapamycin associated protein 1
Alternative Names: Serine/threonine-protein kinase mTOR|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP12-rapamycin complex-associated protein|Mammalian target of rapamycin (mTOR)|Mechanistic target of rapamycin|Rapamycin and FKBP12 target 1|Rapamycin target protein 1|MTOR|FRAP|FRAP1|FRAP2|RAFT1|RAPT1 antibody
UniProt ID: P42345
Observed MW: 290 kDa

Application

Tested Applications: ELISA, WB, IHC
Recommended dilution: WB: 1:200 - 1:500; IHC: 1:100 - 1:200

Validated Images

HeLa cells were subjected to SDS PAGE followed by western blot with FNab05417(MTOR antibody) at dilution of 1:300

Immunohistochemistry of paraffin-embedded human testis using FNab05417(MTOR antibody) at dilution of 1:100

Background: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Identification of Novel Insulin Resistance Related ceRNA Network in T2DM and Its Potential Editing by CRISPR/Cas9

IF: 5.923

Journal:

International Journal of Molecular Sciences

Cited Date:

2021-08-06

Product:

Design, synthesis, anti-inflammatory evaluation, and molecular modelling of new coumarin-based analogs combined curcumin and other heterocycles as potential TNF-α production inhibitors via upregulating Nrf2/HO-1, downregulating AKT/mTOR signalling pathways and downregulating NF-κB in LPS induced macrophages

IF: 5.6

Journal:

Journal of Enzyme Inhibition and Medicinal Chemistry

Author:

Pharmaceutical Chemistry Department, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, 6th of October City, Egypt

Cited Date:

2023-08-25

Product:

MTOR antibody

Vitamin D and lactoferrin attenuate stress-induced colitis in Wistar rats via enhancing AMPK expression with inhibiting mTOR–STAT3 signaling and modulating autophagy

IF: 3.963

Journal:

Cell Biochemistry & Function

Author:

Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Cited Date:

2023-06-23

Product:

MTOR antibody

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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