Products
FEN1 antibody
- Product Name
- FEN1 antibody
- Catalogue No.
- FNab03075
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- flap structure-specific endonuclease 1
- Alternative Names
- Flap endonuclease 1 (FEN-1)|DNase IV|Flap structure-specific endonuclease 1|Maturation factor 1 (MF1 antibody, hFEN-1)|FEN1|RAD2 antibody
- UniProt ID
- P39748
- Observed MW
- 42 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100; IP : 1:50 - 1:200
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab03075(FEN1 antibody) at dilution of 1:1000
IP Result of anti-FEN1 (IP:FNab03075, 4ug; Detection:FNab03075 1:500) with NIH/3T3 cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab03075( FEN1 Antibody) at dilution of 1:100
- Background
- The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
