Products

CD3 epsilon antibody

Synonyms:CD3 antibody, CD3 epsilon antibody, CD3E antibody, CD3-epsilon antibody, T3E antibody, TCRE antibody
Catalogue No.:FNab01459Reativity:Human
Host:MouseTested Application:ELISA, WB, IHC, IF, FC
Clonality:monoclonalIsotype:IgG1
  • SPECIFICATIONS
Product Name
CD3 epsilon antibody
Catalogue No.
FNab01459
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
2E4
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
CD3e molecule, epsilon (CD3-TCR complex)
Alternative Names
CD3 antibody, CD3 epsilon antibody, CD3E antibody, CD3-epsilon antibody, T3E antibody, TCRE antibody
UniProt ID
P07766
Observed MW
23 kDa
Application
Tested Applications
ELISA, WB, IHC, IF, FC
Recommended dilution
WB: 1:2000 - 1:20000; IHC: 1:1000 - 1:10000; IF: 1:50 - 500
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01459( CD3E Antibody) at dilution of 1:10000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab01459( CD3E Antibody) at dilution of 1:8000
Background
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.