Human HS-IL-15(High sensitive Interleukin 15) Accquant ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 5h assay time. The microplate provided in this kit has been precoated with anti IL-15 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-15 bound to precoated antibody. Add HRP-Streptavidin(HRP-SA) and Biotin system (BS). After incubation, wash unbound conjugate with wash buffer. TMB substrate is obtained by amplifying signal twice and used to visualize HRP enzymatic reaction. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-15 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
AQ-H0177-B
Alias: 
IL-15 ELISA Kit, IL15 ELISA Kit, Interleukin 15 ELISA Kit
Species: 
Human
Range: 
0.31-20pg/ml
Sensitivity: 
0.875pg/ml
  • SPECIFICATIONS
Product Name
Human HS-IL-15(High sensitive Interleukin 15) Accquant ELISA Kit
Alias
IL-15 ELISA Kit, IL15 ELISA Kit, Interleukin 15 ELISA Kit
Catalogue No.
AQ-H0177-B
Size
48T/96T
Species
Human
UniProt No.
P40933
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
5 hours
Range
0.31-20pg/ml
Sensitivity
0.875pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with IL-15 , no obvious cross reaction with other analogues.
ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit
E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E0030 Biotin-labeled Antibody 5ml 10ml 2-8°C (Avoid Direct Light)
E0340 HRP-Streptavidin (HRP-SA) 10ml 20ml
E024 TMB Substrate 5ml 10ml
E039 Sample Dilution Buffer 10ml 20ml 2-8°C
E005 assay dilution 2.5ml 5ml
E041 Biotin System (BS, Concentrated, 100X) 60ul 120ul
E042 BS Dilution Buffer 5ml 10ml
E026 Stop Solution 5ml 10ml
E038 Wash Buffer(Concentrated, 25X) 30ml 30ml
E006 Plate Sealer 3 pieces 5 pieces  
E007 Product Description 1 copy 1 copy
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 0.179 0.188 0.183
0.312 0.211 0.219 0.215
0.625 0.357 0.371 0.364
1.25 0.557 0.579 0.568
2.5 0.939 0.977 0.958
5 1.239 1.289 1.264
10 1.815 1.889 1.852
20 2.407 2.505 2.456
AQ-H0177-B Standard Curve Image
Recovery

Add a certain amount of IL-15 into the sample. Calculate the recovery by comparing the measured value with the expected amount of IL-15 in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 94-105 100
EDTA plasma(n=10) 85-97 92
Heparin plasma(n=10) 91-102 96
Linearity

Dilute the sample with a certain amount of IL-15 at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 85-104% 83-94% 83-100%
EDTA plasma(n=10) 85-105% 83-101% 81-98%
Heparin plasma(n=10) 87-103% 86-99% 80-100%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 0.58 2.35 9.72 0.66 2.41 10.2
Standard deviation 0.03 0.13 0.41 0.03 0.11 0.4
CV(%) 5.92 5.72 4.23 5.08 4.62 3.95
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100