Recombinant Human Osteopontin

Alternative Names:BNSP Protein, Bone sialoprotein 1 Protein, BSPI Protein, ETA 1 Protein, Nephropontin Protein, OPN Protein, Osteopontin Protein, secreted phosphoprotein 1 Protein, SPP 1 Protein, SPP1 Protein, Urinary stone protein Protein, Uropontin Protein
Catalogue No.:P9424Uniprot ID:P10451
Host:E.ColiExpression Region:17-314
Mol. Weight:60-65 kDaTags:C-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human Osteopontin
Catalogue No.
P9424
Species
Human
Host
E.Coli
Expression Region
17-314
Mol. Weight
60-65 kDa
Tags
C-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
BNSP Protein, Bone sialoprotein 1 Protein, BSPI Protein, ETA 1 Protein, Nephropontin Protein, OPN Protein, Osteopontin Protein, secreted phosphoprotein 1 Protein, SPP 1 Protein, SPP1 Protein, Urinary stone protein Protein, Uropontin Protein
SDS-PAGE