Recombinant Human MDM2

Alternative Names:ACTFS Protein, Double minute 2 protein Protein, E3 ubiquitin-protein ligase Mdm2 Protein, Hdm 2 Protein, Hdm2 Protein, HDMX Protein, MDM 2 Protein, MDM2 Protein, MDM2 oncogene E3 ubiquitin protein ligase Protein, Mdm2 p53 E3 ubiquitin protein ligase homolog Protein, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa Protein
Catalogue No.:P5612Uniprot ID:Q00987-11
Host:E.ColiExpression Region:1-118
Mol. Weight:15.4 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human MDM2
Catalogue No.
P5612
Species
Human
Host
E.Coli
Expression Region
1-118
Mol. Weight
15.4 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
ACTFS Protein, Double minute 2 protein Protein, E3 ubiquitin-protein ligase Mdm2 Protein, Hdm 2 Protein, Hdm2 Protein, HDMX Protein, MDM 2 Protein, MDM2 Protein, MDM2 oncogene E3 ubiquitin protein ligase Protein, Mdm2 p53 E3 ubiquitin protein ligase homolog Protein, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa Protein
SDS-PAGE