Recombinant Rat CCL4

Alternative Names:MIP 1 beta Protein, Secreted protein G 26 Protein, ACT 2 Protein, ACT-2 Protein, ACT2 Protein, AT744.1 Protein, AT744.2 Protein, C C motif chemokine 4 Protein, C C motif chemokine 4 like Protein, C C motif chemokine ligand 4 like 1 Protein, C C motif chemokine ligand 4 like 2 Protein, CC chemokine ligand 4 Protein, CC chemokine ligand 4L1 Protein, CC chemokine ligand 4L1d2 Protein
Catalogue No.:P5301Uniprot ID:P50230
Host:E.ColiExpression Region:24-92
Mol. Weight:7.5 kDaTags:N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
  • SPECIFICATIONS
Product Name
Recombinant Rat CCL4
Catalogue No.
P5301
Species
Rat
Host
E.Coli
Expression Region
24-92
Mol. Weight
7.5 kDa
Tags
N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 10-30 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
MIP 1 beta Protein, Secreted protein G 26 Protein, ACT 2 Protein, ACT-2 Protein, ACT2 Protein, AT744.1 Protein, AT744.2 Protein, C C motif chemokine 4 Protein, C C motif chemokine 4 like Protein, C C motif chemokine ligand 4 like 1 Protein, C C motif chemokine ligand 4 like 2 Protein, CC chemokine ligand 4 Protein, CC chemokine ligand 4L1 Protein, CC chemokine ligand 4L1d2 Protein