Recombinant Human TFIIE alpha

Alternative Names:FE Protein, General transcription factor IIE 56 kDa subunit Protein, General transcription factor IIE subunit 1 Protein, General transcription factor IIE Protein, polypeptide 1 Protein, alpha 56kDa Protein, Gtf2e1 Protein, MAL7P1.86 Protein, T2EA_HUMAN Protein, TF2E1 Protein, TFIIE A Protein, TFIIE alpha Protein, TFIIE-alpha Protein, Transcription initiation factor IIE alpha subunit Protein
Catalogue No.:P5029Uniprot ID:P29083
Host:E.ColiExpression Region:1-439
Mol. Weight:48.2 kDaTags:N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
  • SPECIFICATIONS
Product Name
Recombinant Human TFIIE alpha
Catalogue No.
P5029
Species
Human
Host
E.Coli
Expression Region
1-439
Mol. Weight
48.2 kDa
Tags
N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
FE Protein, General transcription factor IIE 56 kDa subunit Protein, General transcription factor IIE subunit 1 Protein, General transcription factor IIE Protein, polypeptide 1 Protein, alpha 56kDa Protein, Gtf2e1 Protein, MAL7P1.86 Protein, T2EA_HUMAN Protein, TF2E1 Protein, TFIIE A Protein, TFIIE alpha Protein, TFIIE-alpha Protein, Transcription initiation factor IIE alpha subunit Protein