DNA directed RNA polymerase II A Protein, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit Protein, DNA-directed RNA polymerase II subunit A Protein, DNA-directed RNA polymerase II subunit RPB1 Protein, DNA-directed RNA polymerase III largest subunit Protein, hRPB220 Protein, hsRPB1 Protein, POLR2 Protein
Catalogue No.:
P4965
Uniprot ID:
P24928
Host:
E.Coli
Expression Region:
1586-1951
Mol. Weight:
55 kDa
Tags:
N-terminal His Tag
SPECIFICATIONS
Product Name
Recombinant Human RNA polymerase II CTD repeat YSPTSPS
Catalogue No.
P4965
Species
Human
Host
E.Coli
Expression Region
1586-1951
Mol. Weight
55 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
DNA directed RNA polymerase II A Protein, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit Protein, DNA-directed RNA polymerase II subunit A Protein, DNA-directed RNA polymerase II subunit RPB1 Protein, DNA-directed RNA polymerase III largest subunit Protein, hRPB220 Protein, hsRPB1 Protein, POLR2 Protein