Recombinant Human Desmoplakin

Alternative Names:250/210 kDa paraneoplastic pemphigus antigen Protein, Desmoplakin I Protein, Desmoplakin II Protein, DP I Protein, DP II Protein, DPI Protein, DPII Protein, DSP Protein, KPPS2 Protein, PPKS2 Protein
Catalogue No.:P4793Uniprot ID:P15924
Host:E.ColiExpression Region:78-300
Mol. Weight:24.4 kDaTags:N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
  • SPECIFICATIONS
Product Name
Recombinant Human Desmoplakin
Catalogue No.
P4793
Species
Human
Host
E.Coli
Expression Region
78-300
Mol. Weight
24.4 kDa
Tags
N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
250/210 kDa paraneoplastic pemphigus antigen Protein, Desmoplakin I Protein, Desmoplakin II Protein, DP I Protein, DP II Protein, DPI Protein, DPII Protein, DSP Protein, KPPS2 Protein, PPKS2 Protein