Recombinant Human IL-4

Alternative Names:B cell growth factor 1 Protein, B cell IgG differentiation factor Protein, B Cell Stimulatory Factor 1 Protein, B-cell stimulatory factor 1 Protein, BCGF 1 Protein, BCGF1 Protein, Binetrakin Protein, BSF-1 Protein, BSF1 Protein, IGG1 induction factor Protein, IL 4 Protein, IL-4 Protein, IL4 Protein, IL4_HUMAN Protein, Il4e12 Protein, Interleukin 4 Protein, Interleukin 4 variant 2 Protein, Interleukin 4 Protein
Catalogue No.:P4505Uniprot ID:P05112
Host:E.ColiExpression Region:25-153
Mol. Weight:14.1 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human IL-4
Catalogue No.
P4505
Species
Human
Host
E.Coli
Expression Region
25-153
Mol. Weight
14.1 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
B cell growth factor 1 Protein, B cell IgG differentiation factor Protein, B Cell Stimulatory Factor 1 Protein, B-cell stimulatory factor 1 Protein, BCGF 1 Protein, BCGF1 Protein, Binetrakin Protein, BSF-1 Protein, BSF1 Protein, IGG1 induction factor Protein, IL 4 Protein, IL-4 Protein, IL4 Protein, IL4_HUMAN Protein, Il4e12 Protein, Interleukin 4 Protein, Interleukin 4 variant 2 Protein, Interleukin 4 Protein
SDS-PAGE