Recombinant Mouse IL-1 alpha

Alternative Names:BAF Protein, FAF Protein, Hematopoietin 1 Protein, Hematopoietin-1 Protein, IL 1 alpha Protein, IL 1A Protein, IL-1 alpha Protein, Il-1a Protein, IL1 Protein, IL1 ALPHA Protein, IL1A Protein, IL1A_HUMAN Protein, IL1F1 Protein, Interleukin 1 alpha Protein, Interleukin-1 alpha Protein, Interleukin1 alpha Protein, LAF Protein, LEM Protein, Preinterleukin 1 alpha Protein, Pro interleukin 1 alpha Protein
Catalogue No.:P4424Uniprot ID:P01582
Host:E.ColiExpression Region:115-270
Mol. Weight:17.1 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Mouse IL-1 alpha
Catalogue No.
P4424
Species
Mouse
Host
E.Coli
Expression Region
115-270
Mol. Weight
17.1 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is 10-50 pg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
BAF Protein, FAF Protein, Hematopoietin 1 Protein, Hematopoietin-1 Protein, IL 1 alpha Protein, IL 1A Protein, IL-1 alpha Protein, Il-1a Protein, IL1 Protein, IL1 ALPHA Protein, IL1A Protein, IL1A_HUMAN Protein, IL1F1 Protein, Interleukin 1 alpha Protein, Interleukin-1 alpha Protein, Interleukin1 alpha Protein, LAF Protein, LEM Protein, Preinterleukin 1 alpha Protein, Pro interleukin 1 alpha Protein
SDS-PAGE