Recombinant Human TGF alpha

Alternative Names:EGF like TGF Protein, EGF-like TGF Protein, ETGF Protein, TFGA Protein, TGF A Protein, TGF type 1 Protein, TGF-alpha Protein, Tgfa Protein, TGFA_HUMAN Protein, Transforming growth factor alpha Protein, Transforming growth factor alpha precursor Protein, Wa1 Protein, Waved 1 Protein
Catalogue No.:P4400Uniprot ID:P01135
Host:E.ColiExpression Region:40-89
Mol. Weight:26.2 kDaTags:N-terminal His-IF2DI Tag
  • SPECIFICATIONS
Product Name
Recombinant Human TGF alpha
Catalogue No.
P4400
Species
Human
Host
E.Coli
Expression Region
40-89
Mol. Weight
26.2 kDa
Tags
N-terminal His-IF2DI Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.6-1 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
EGF like TGF Protein, EGF-like TGF Protein, ETGF Protein, TFGA Protein, TGF A Protein, TGF type 1 Protein, TGF-alpha Protein, Tgfa Protein, TGFA_HUMAN Protein, Transforming growth factor alpha Protein, Transforming growth factor alpha precursor Protein, Wa1 Protein, Waved 1 Protein
SDS-PAGE