Recombinant Human IGF-1

Alternative Names:IBP1 Protein, IGF I Protein, IGF IA Protein, IGF IB Protein, IGF-I Protein, Igf1 Protein, IGF1_HUMAN Protein, IGF1A Protein, IGFI Protein, IGFIA Protein, Insulin like growth factor 1 Protein, Insulin like growth factor 1 (somatomedin C) Protein, Insulin like growth factor IA Protein, Insulin like growth factor IB Protein, Insulin-like growth factor I Protein, Mechano growth factor Protein, MGF Protein
Catalogue No.:P2663Uniprot ID:P05019
Host:E.ColiExpression Region:49-118
Mol. Weight:28.3 kDaTags:N-terminal His-IF2DI Tag
  • SPECIFICATIONS
Product Name
Recombinant Human IGF-1
Catalogue No.
P2663
Species
Human
Host
E.Coli
Expression Region
49-118
Mol. Weight
28.3 kDa
Tags
N-terminal His-IF2DI Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.6-2 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
IBP1 Protein, IGF I Protein, IGF IA Protein, IGF IB Protein, IGF-I Protein, Igf1 Protein, IGF1_HUMAN Protein, IGF1A Protein, IGFI Protein, IGFIA Protein, Insulin like growth factor 1 Protein, Insulin like growth factor 1 (somatomedin C) Protein, Insulin like growth factor IA Protein, Insulin like growth factor IB Protein, Insulin-like growth factor I Protein, Mechano growth factor Protein, MGF Protein
SDS-PAGE