Recombinant Human TIMP-1

Alternative Names:CLGI Protein, Collagenase inhibitor Protein, EPA Protein, EPO Protein, HCI Protein, Metalloproteinase inhibitor 1 Protein, TIMP Protein, TIMP 1 Protein, TIMP1 Protein
Catalogue No.:P2407Uniprot ID:P01033
Host:HEK293 cellsExpression Region:24-207
Mol. Weight:28 kDaTags:C-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human TIMP-1
Catalogue No.
P2407
Species
Human
Host
HEK293 cells
Expression Region
24-207
Mol. Weight
28 kDa
Tags
C-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2. The IC50 value is < 5 nRecombinant Mouse
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
CLGI Protein, Collagenase inhibitor Protein, EPA Protein, EPO Protein, HCI Protein, Metalloproteinase inhibitor 1 Protein, TIMP Protein, TIMP 1 Protein, TIMP1 Protein
SDS-PAGE