Products
Recombinant Human GLO1
- Product Name
- Recombinant Human GLO1
- Catalogue No.
- P1198
- Species
- Human
- Host
- E.Coli
- Expression Region
- 2-184
- Mol. Weight
- 22.5 KDa
- Tags
- N-terminal His Tag
- Purity
- Greater than 95% as determined by SDS-PAGE
- Application
- Western Blot, ELISA
- Activity
- Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione
- Formulation
- Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- Aldoketomutase Protein, glo1 Protein, GLOD1 Protein, Glx I Protein, GLYI Protein, glyoxalase domain containing 1 Protein, Glyoxalase I Protein, Ketone aldehyde mutase Protein, Ketone-aldehyde mutase Protein, Lactoyl glutathione lyase Protein, Lactoylglutathione lyase Protein, LGUL_HUMAN Protein, Methylglyoxalase Protein, S D lactoylglutathione methylglyoxal lyase Protein, S-D-lactoylglutathione methylglyoxal lyase Protein
- SDS-PAGE
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