Recombinant Human Vinculin

Alternative Names:CMD1W Protein, Metavinculin Protein, MVCL Protein, VCL Protein, Vinculin Protein
Catalogue No.:P0252Uniprot ID:P18206-2
Host:E.ColiExpression Region:777-1066
Mol. Weight:60.5 KDaTags:N-terminal GST tag
  • SPECIFICATIONS
Product Name
Recombinant Human Vinculin
Catalogue No.
P0252
Species
Human
Host
E.Coli
Expression Region
777-1066
Mol. Weight
60.5 KDa
Tags
N-terminal GST tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
CMD1W Protein, Metavinculin Protein, MVCL Protein, VCL Protein, Vinculin Protein
SDS-PAGE