Products
Purified Anti-Mouse CD16/32 Antibody(2.4G2)
| Synonyms： | CD16a/b|CD32|CD32A/B|FCG2A|FCGR2A/BFCGR3|FCGR3A/B|Fc fragment of IgG low affinity IIIa/b receptor|Fc fragment of IgG low affinity IIIb receptor|Fc fragment of IgG low affinity IIa/b receptor|Fc gamma RIIa/b |Fc gamma receptor III A/B|FcGR|CD16/32 antibody | ||
| Catalogue No.： | FNab30106 | Reactivity： | Mouse |
| Host： | Rat | Tested Application： | FC, Block |
| Clonality： | monoclonal | Isotype： | IgG2b, kappa |
- SPECIFICATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- Purified Anti-Mouse CD16/32 Antibody(2.4G2)
- Catalogue No.
- FNab30106
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥98% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2b, kappa
- Clone ID
- 2.4G2
- Storage
- Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. Store at 2-8°C valid for 12 months or -20°C valid for long term storage, avoid freeze/thaw cycles.
- Immunogen
- Recombinant Mouse CD16/32 protein
- Alternative Names
- CD16a/b|CD32|CD32A/B|FCG2A|FCGR2A/BFCGR3|FCGR3A/B|Fc fragment of IgG low affinity IIIa/b receptor|Fc fragment of IgG low affinity IIIb receptor|Fc fragment of IgG low affinity IIa/b receptor|Fc gamma RIIa/b |Fc gamma receptor III A/B|FcGR|CD16/32 antibody
- UniProt ID
- P08508|P08101
- Tested Applications
- FC, Block
- Recommended dilution
- The suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Background
- CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).