anti- PARP1 antibody
FNab09939
Catalogue No.
- Synonyms:
- ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
- Host:
- Mouse
- Reactivity:
- Human, Mouse, Rat
- Isotype:
- IgG1
- Tested Application:
- ELISA, WB, IHC, IF
Product Information
- Product Name
- anti- PARP1 antibody
- Catalogue No.
- FNab09939
- Size
- 100µg
- Form
- liquid
- Purification
- protein A+G purified
- Purity
- ≥95% as determined by SDS-PAGE
- Host
- Mouse
- Clonality
- monoclonal
- Isotype
- IgG1
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
- PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.
Immunogen Information
- Immunogen
- poly (ADP-ribose) polymerase 1
- Synonyms
- ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
- Observed MW
- 115 kDa, 89 kDa
Application
- Tested Application
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:5000 - 1:30000; IHC: 1:100 - 1:1000; IF: 1:50 - 1:500
Uniprot information
- UniProt ID
- P09874
Images
- Image 1
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Immunohistochemistry of paraffin-embedded human breast cancer using FNab09939(PARP1 antibody) at dilution of 1:600
- Image 2
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Immunofluorescent analysis of HeLa cells using FNab09939 (PARP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
- Image 4
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab09939(PARP1 Antibody) at dilution of 1:20000