Products
YTHDC1 antibody
- Product Name
- YTHDC1 antibody
- Catalogue No.
- FNab09571
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- YTH domain containing 1
- Alternative Names
- KIAA1966 antibody, Putative splicing factor YT521 antibody, YT521 antibody, YT521 B antibody, YTH domain containing 1 antibody, YTHDC1 antibody
- UniProt ID
- Q96MU7
- Observed MW
- 110 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:200-1:1000
Validated Images
MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab09571(YTHDC1 Antibody) at dilution of 1:300
- Background
- Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine(m6A)-containing RNAs(PubMed:26318451, PubMed:26876937, PubMed:25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10(PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing(PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing(PubMed:26876937). May also regulate alternative splice site selection(PubMed:20167602).
