Products
USP16 antibody
- Product Name
- USP16 antibody
- Catalogue No.
- FNab09312
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ubiquitin specific peptidase 16
- Alternative Names
- Deubiquitinating enzyme 16 antibody, Ubiquitin thiolesterase 16 antibody, UBP M antibody, USP16 antibody
- UniProt ID
- Q9Y5T5
- Observed MW
- 94 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09312(USP16 antibody) at dilution of 1:1000
IP Result of anti-USP16 (IP:FNab09312, 5ug; Detection:FNab09312 1:1000) with HeLa cells lysate 880ug.
Immunohistochemistry of paraffin-embedded human kidney using FNab09312(USP16 antibody) at dilution of 1:100
- Background
- Specifically deubiquitinates 'Lys-120' of histone H2A(H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3(H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B-and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
