Products
UIMC1 antibody
- Product Name
- UIMC1 antibody
- Catalogue No.
- FNab09247
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ubiquitin interaction motif containing 1
- Alternative Names
- BRCA1 A complex subunit RAP80 antibody, RAP80 antibody, Receptor associated protein 80 antibody, RXRIP110 antibody, UIMC1 antibody, X2HRIP110 antibody
- UniProt ID
- Q96RL1
- Observed MW
- 97 kDa,80 kDa,71-75 kDa,61 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC,IF
- Recommended dilution
- WB: 1:200-1:1000; IP: 1：200-1：2000；IHC: 1：20-1：200; IF: 1：10-1：100
Validated Images
mouse colon tissue were subjected to SDS PAGE followed by western blot with FNab09247(UIMC1 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab09247(UIMC1 Antibody) at dilution of 1:50
- Background
- Ubiquitin-binding protein(PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin(PubMed:19328070, Ref.36). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
