Products
SETMAR antibody
- Product Name
- SETMAR antibody
- Catalogue No.
- FNab07773
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- SET domain and mariner transposase fusion gene
- UniProt ID
- Q53H47
- Observed MW
- 77 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
HL-60 cells were subjected to SDS PAGE followed by western blot with FNab07773( SETMAR Antibody) at dilution of 1:600
IP Result of anti-SETMAR (IP:FNab07773, 4ug; Detection:FNab07773 1:500) with HL-60 cells lysate 1600ug.
- Background
- Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats(TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity(PubMed:16332963, PubMed:16672366, PubMed:17877369, PubMed:17403897, PubMed:18263876, PubMed:22231448, PubMed:24573677, PubMed:20521842). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining(PubMed:16332963, PubMed:21187428, PubMed:22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A(PubMed:18790802, PubMed:20457750).
