Products
SETD2 antibody
- Product Name
- SETD2 antibody
- Catalogue No.
- FNab07767
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- SET domain containing 2
- Alternative Names
- HIF1 antibody, HYPB antibody, KIAA1732 antibody, KMT3A antibody, SET2 antibody
- UniProt ID
- Q9BYW2
- Observed MW
- 287 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
H460 cells were subjected to SDS PAGE followed by western blot with FNab07767(SETD2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat brain tissue slide using FNab07767(SETD2 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using FNab07767 (SETD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
- Background
- Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3(H3K36me3) using dimethylated 'Lys-36'(H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
