Products
SART3 antibody
- Product Name
- SART3 antibody
- Catalogue No.
- FNab07612
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- squamous cell carcinoma antigen recognized by T cells 3
- Alternative Names
- DSAP1 antibody, hSART 3 antibody, KIAA0156 antibody, P100 antibody, p110 antibody, p110(nrb) antibody, RP11 13G14 antibody, SART 3 antibody, SART3 antibody, TIP110 antibody, antibody
- UniProt ID
- Q15020
- Observed MW
- 110 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07612(SART3 antibody) at dilution of 1:1000
IP Result of anti-SART3 (IP: FNab07612, 4ug; Detection: FNab07612 1:800) with HeLa cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis using FNab07612(SART3 antibody) at dilution of 1:100
- Background
- U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation(PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites(PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly(PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair(PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC(By similarity).
