Products
PSME4 antibody
- Product Name
- PSME4 antibody
- Catalogue No.
- FNab06897
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- proteasome(prosome, macropain) activator subunit 4
- Alternative Names
- KIAA0077 antibody
- UniProt ID
- Q14997
- Observed MW
- 140 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000 ; IHC: 1:50-1:500; IF: 1:10-1:100
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06897(PSME4 antibody) at dilution of 1:400
IP Result of anti-PSME4 (IP:FNab06897, 3ug; Detection:FNab06897 1:500) with HEK-293 cells lysate 1760ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab06897( PSME4 antibody) at dilution of 1:200
- Background
- Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP-and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like(BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
