Products

PRKAR1A antibody

Synonyms:PKR1 antibody, PRKAR1 antibody, TSE1 antibody
Catalogue No.:FNab06779Reativity:Human
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
PRKAR1A antibody
Catalogue No.
FNab06779
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
Alternative Names
PKR1 antibody, PRKAR1 antibody, TSE1 antibody
UniProt ID
P10644
Observed MW
45 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab06779(PRKAR1A antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human oophoroma using FNab06779(PRKAR1A antibody) at dilution of 1:100
Background
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.