Products
PMS2 antibody
- Product Name
- PMS2 antibody
- Catalogue No.
- FNab06579
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 4B8
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- PMS2 postmeiotic segregation increased 2
- Alternative Names
- HNPCC4 antibody, PMS1 protein homolog 2 antibody, PMS2 antibody, PMS2CL antibody, PMSL2 antibody
- UniProt ID
- P54278
- Observed MW
- 100KD
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:50-1:500
Validated Images
A431 cells were subjected to SDS PAGE followed by western blot with FNab06579(PMS2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using FNab06579(PMS2 Antibody) at dilution of 1:400
- Background
- Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
