Products
MSH6 antibody
- Product Name
- MSH6 antibody
- Catalogue No.
- FNab05376
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 4C4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mutS homolog 6
- Alternative Names
- G/T mismatch binding protein antibody, GTBP antibody, GTMBP antibody, hMSH6 antibody, HNPCC5 antibody, HSAP antibody, MSH6 antibody, MutS alpha 160 kDa subunit antibody, mutS homolog 6 (E. coli) antibody, p160 antibody
- UniProt ID
- P52701
- Observed MW
- 160 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05376(MSH6 Antibody) at dilution of 1:1000
IP Result of anti-MSH6 (IP:FNab05376, 4ug; Detection:FNab05376 1:600) with HEK-293 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab05376(MSH6 Antibody) at dilution of 1:100
- Background
- Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3(H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
