Products
HINT1 antibody
- Product Name
- HINT1 antibody
- Catalogue No.
- FNab03866
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- histidine triad nucleotide binding protein 1
- Alternative Names
- HINT antibody, PKCI1 antibody, PRKCNH1 antibody
- UniProt ID
- P49773
- Observed MW
- 14 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab03866(HINT1 antibody) at dilution of 1:400
IP Result of anti-HINT1 (IP:FNab03866, 3ug; Detection:FNab03866 1:500) with mouse brain tissue lysate 7500ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab03866(HINT1 Antibody) at dilution of 1:400 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate(AMP-NH2), adenosine 5'monophosphomorpholidate(AMP-morpholidate) and guanosine 5'monophosphomorpholidate(GMP-morpholidate). Hydrolyzes lysyl-AMP(AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP(GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF(SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
