Products
Hemoglobin alpha antibody
- Product Name
- Hemoglobin alpha antibody
- Catalogue No.
- FNab03825
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- hemoglobin, alpha 1
- Alternative Names
- Alpha globin antibody, HBA1 antibody, HBA2 antibody, Hemoglobin a antibody, Hemoglobin alpha chain antibody, Hemoglobin subunit alpha antibody, hemoglobin antibody, alpha 1 antibody
- UniProt ID
- P69905
- Observed MW
- 13 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Validated Images
mouse liver were subjected to SDS PAGE followed by western blot with FNab03825(Hemoglobin alpha antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse heart using FNab03825(Hemoglobin alpha antibody) at dilution of 1:100
- Background
- The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
