Products
HA-Tag antibody
- Product Name
- HA-Tag antibody
- Catalogue No.
- FNab03762
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 6A3
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- HA tag
Application
- Tested Applications
- ELISA, IP, WB, IF
- Recommended dilution
- WB: 1:5000-1:50000; IP: 1:2000-1:20000; IF: 1:10-1:100
Validated Images
HA-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03762(HA tag Antibody) at dilution of 1:20000
IP Result of anti-HA-tag (IP: FNab03762, 5ug; Detection: FNab03762 1:5000) with Transfected HEK-293 cells lysate 400ug.
- Background
- The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
